Tris Buffered Saline with Tween ® 20 (TBST-10X) from Cell Signaling Technology

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ItemTris Buffered Saline with Tween ® 20 (TBST-10X)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number9997S
Quantity1000 ml
Research AreaRelated Products

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Cell Signaling Technology

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Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
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Website: http://www.cellsignal.com/

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(1) Cationic LNP-formulated mRNA expressing Tie2-agonist in the lung endothelium prevents pulmonary vascular leakageMolecular Therapy - Nucleic AcidsDecember 12, 2023Katrin Radloff, Birgitt Gutbier, Charlotte Maeve Dunne, Hanieh Moradian, Marko Schwestka, Manfred Gossen, Katharina Ahrens, Laura Kneller, Yadong Wang, Akanksha Moga, et al.washed with Tris-buffered saline with Tween 20 (Cell Signaling Technology, Danvers, MA, catalog no. 9997S) and probed at 4°C overnight with specific primary antibodies diluted in Intercept Antibody Diluent

(2) HDAC3 aberration-incurred GPX4 suppression drives renal ferroptosis and AKI-CKD progressionRedox BiologyDecember 1, 2023Lijun Zhang, Fang Chen, Jian Dong, Rong Wang, Guangyu Bi, Daoliang Xu, Yingwei Zhang, Yijun Deng, Wenjun Lin, Zhongzhou Yang, et al.% bovine serum albumin (BSA, 4240GR500, BioFroxx, Germany) in Tris-buffered saline-Tween 20 (TBST, 9997, CST, USA) and then incubated with primary antibodies. The anti-bodies used were as following: GPX4

(3) Mitochondrial-targeted antioxidant attenuates preeclampsia-like phenotypes induced by syncytiotrophoblast-specific Gαq signalingSci AdvDecember 1, 2023Megan A. Opichka, M. Christine Livergood, Kirthikaa Balapattabi, McKenzie L. Ritter, Daniel T. Brozoski, Kelsey K. Wackman, Ko-Ting Lu, Kaleigh N. Kozak, Clive Wells, Agnes B. Fogo, et al.5% normal goat serum (Cell Signaling, 5425) in tris-buffered saline with Tween 20 (Cell Signaling, 9997) for 1 hour. CD31 rabbit monoclonal antibody (Cell Signaling, 77699, 1:100) in antibody diluent (Cell Signaling

(4) Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitationF1000ResearchSeptember 29, 2023Riham Ayoubi, Kathleen Southern, Carl Laflamme, NeuroSGC/YCharOS collaborative group(Wisent, cat. number 800-095) in TBS with 0.1% Tween 20 (TBST) (Cell Signaling Technology, cat. number 9997). Following three washes with TBST, the peroxidase conjugated secondary antibody was incubated at a

(5) Elucidation of E3 ubiquitin ligase specificity through proteome-wide internal degron mappingMolecular CellSeptember 21, 2023Zhiqian Zhang, Brandon Sie, Aiquan Chang, Yumei Leng, Christopher Nardone, Richard T. Timms, Stephen J. Elledgethe gel to a 0.2 𝜇m nitrocellulose membrane (BioRad 1704158). Membranes were blocked in 1x TBST (CST 9997S) with 5% milk for 30 min at room temperature. Then rabbit anti-mTOR (CST 2983S), rabbit anti-FLAG (CST

(6) The Autoantibody Array Assay: A Novel Autoantibody Detection MethodDiagnostics (Basel)September 13, 2023Yuta Norimatsu, Kazuki Mitsuru Matsuda, Kei Yamaguchi, Chihiro Ono, Taishi Okumura, Emi Kogo, Hirohito Kotani, Teruyoshi Hisamoto, Ai Kuzumi, Takemichi Fukasawa, et al.at room temperature for 30 min and washed with Tris-buffered saline containing 0.1% Tween 20 (TBST; 9997S, Cell Signaling Technology, Danvers, MA, USA). The plates were then incubated in blocking buffer (50

(7) Identification of high-performing antibodies for Superoxide dismutase [Cu-Zn] 1 (SOD1) for use in Western blot, immunoprecipitation, and immunofluorescenceF1000ResearchAugust 31, 2023Riham Ayoubi, Walaa Alshafie, Zhipeng You, Kathleen Southern, Peter S. McPherson, Carl Laflamme(Wisent, cat. number 800-095) in TBS with 0,1% Tween 20 (TBST) (Cell Signaling Technology, cat. number 9997). Following three washes with TBST, the peroxidase conjugated secondary antibody was incubated at a

(8) Comprehensive autoantibody profiling in systemic autoimmunity by a highly-sensitive multiplex protein arrayFrontiers in ImmunologyAugust 28, 2023Ai Kuzumi, Yuta Norimatsu, Kazuki M. Matsuda, Chihiro Ono, Taishi Okumura, Emi Kogo, Naoki Goshima, Takemichi Fukasawa, Natsumi Fushida, Motoki Horii, et al.at room temperature for 30 min and washed with Tris-buffered saline containing 0.1% Tween 20 (TBST; 9997S, Cell Signaling Technology, Danvers, MA, USA). The plates were then incubated in blocking buffer (50

(9) Mangiferin and Taurine Ameliorate MSRV Infection by Suppressing NF-κB SignalingMicrobiology SpectrumAugust 17, 2023Caijiao Dai, Li Yu, Zhiwen Wang, Peng Deng, Lijuan Li, Zemao Gu, Xugang He, Jianghua Wang, Junfa Yuanmilk in Tris-buffered saline supplemented with 0.1% Tween 20 (TBST) (Cell Signaling Technology; no. 9997) at 4°C overnight, and then washed with TBST (TBS containing 0.1% Tween 20) buffer for 3 × 10 min. The

(10) TEQUILA-seq: a versatile and low-cost method for targeted long-read RNA sequencingNat CommunAugust 8, 2023Feng Wang, Yang Xu, Robert Wang, Beatrice Zhang, Noah Smith, Amber Notaro, Samantha Gaerlan, Eric Kutschera, Kathryn E. Kadash-Edmondson, Yi Xing, et al.#170-4272). The membranes were blocked with 5% non-fat milk in 1× TBST (Cell Signaling Technology, #9997) at room temperature for 1 h and probed at 4 °C overnight with a 1:1000 dilution of Phospho-Upf1 (Ser1127) antibody

(11) The identification of high-performing antibodies for Coiled-coil-helix-coiled-coil-helix domain containing protein 10 (CHCHD10) for use in Western Blot, immunoprecipitation and immunofluorescenceF1000ResearchJuly 26, 2023Riham Ayoubi, Walaa Alshafie, Kathleen Southern, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group(Wisent, cat. number 800-095) in TBS with 0,1% Tween 20 (TBST) (Cell Signaling Technology, cat. number 9997). Following three washes with TBST, the peroxidase conjugated secondary antibody was incubated at a

(12) Use of the dTAG system in vivo to degrade CDK2 and CDK5 in adult mice and explore potential safety liabilitiesToxicological SciencesJune 28, 2023Paul Yenerall, Tae Sung, Kiran Palyada, Jessie Qian, Seda Arat, Steven W Kumpf, Shih-Wen Wang, Kathleen Biddle, Carlos Esparza, Stephanie Chang, et al.97166). The next morning, membranes were washed 3 times for 5 min using TBST (Cell Signaling, catalog no. 9997), then secondary antibodies were diluted into LiCor Intercept T20 Antibody Diluent (Fisher, catalog

(13) The identification of high-performing antibodies for transmembrane protein 106B (TMEM106B) for use in Western blot, immunoprecipitation, and immunofluorescenceF1000ResearchMarch 21, 2023Riham Ayoubi, Maryam Fotouhi, Kathleen Southern, Ritika Bhajiawala, Rebeka Fanti, Panagiotis Prinos, Peter S. McPherson, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group, ABIF Consortium(Wisent, cat. number 800-095) in TBS with 0,1% Tween 20 (TBST) (Cell Signaling Technology, cat. number 9997). Following three washes with TBST, the peroxidase conjugated secondary antibody was incubated at a

(14) Proximity biotinylation to define the local environment of the protein kinase A catalytic subunit in adrenal cellsSTAR protocolsMarch 17, 2023Omar MH, Lauer SM, Lau HT, Golkowski M, Ong SE, Scott JD.μm,VWR,89508-342 DAPI (1 mg/mL); 1:5000 dilution,Life Technologies,62248 TBST (10×),Cell Signaling Technologies,9997S Sodium hydroxide,Fisher,S318-500 Hydrochloric acid,Fisher,A144S-500 0.2 μm sterile filter,Fisher,09-741-04 GemCell

(15) CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in miceSTAR ProtocolsMarch 1, 2023Annelise Cassidy, Stephane PelletierS staining and imaging, wash nitrocellulose membrane with 25 mL 1× TBST (Cell Signaling Technology, 9997, 137 mM Sodium Chloride, 20 mM Tris, 0.1% Tween-20, pH 7.6) for 5 min at room temperature.h.Incubate

(16) Targeting UGCG Overcomes Resistance to Lysosomal Autophagy InhibitionCancer discoveryFebruary 6, 2023Jain V, Harper SL, Versace AM, Fingerman D, Brown GS, Bhardwaj M, Crissey MAS, Goldman AR, Ruthel G, Liu Q, et al.(SAB3500048, Sigma-Aldrich); PPT1 (TA800501, Origene). PVDF membranes were washed with 1X TBS-T (CS-9997, Cell Signaling Technology, Inc.) and incubated for 1 h at room temperature with species-specific horseradish

(17) Identification of disease-related aberrantly spliced transcripts in myeloma and strategies to target these alterations by RNA-based therapeuticsBlood Cancer JournalFebruary 3, 2023Daisuke Ogiya, Zuzana Chyra, Sigitas J. Verselis, Morgan O’Keefe, Jacquelyn Cobb, Ivane Abiatari, Srikanth Talluri, Anjana Anilkumar Sithara, Teru Hideshima, Michael P. Chu, et al.(Millipore, Bedford, MA). Nonspecific binding was blocked by 5% BSA/0.1% Tween 20 PBS blocking buffer (#9997, Cell Signaling Technology, Danvers, MA). The membrane was incubated with anti-HMMR antibodies from

(18) Euphejolkinolide A, a new ent-abietane lactone from Euphorbia peplus L. with promising biological activity in activating the autophagy-lysosomal pathwayHeliyonFebruary 1, 2023Ran X, Lu QY, Li YY, Pu XX, Guo Y, Yuan MR, Guan SP, Sun M, Jiao L, Yao YG, et al.respectively. The membrane was then washed 3 times with TBST (Tris buffered saline [Cell Signaling Technology, 9997] with 0.1% Tween 20 [Sigma, P1379]) for 5 min each time, and then incubated with either peroxidase-conjugated

(19) New Monoterpenoid Indole Alkaloids from Tabernaemontana crassa Inhibit β-Amyloid42 Production and Phospho-Tau (Thr217)International journal of molecular sciencesJanuary 12, 2023Li S, Han LL, Huang KP, Ma YH, Guo LL, Guo Y, Ran X, Yao YG, Hao XJ, Luo R, et al.abs130996). The membranes were washed 3 times with TBST (Tris-buffered saline (Cell Signaling Technology, 9997) with Tween 20 (0.1%; Sigma, P1379, St. Louis, MO, USA))—each time for 5 min—and subsequently incubated

(20) Engineering human JMJD2A tudor domains for an improved understanding of histone peptide recognitionProteinsJanuary 1, 2023Parkinson J, Hard R, Ainsworth R, Wang W.tudor domain. The array was pre-blocked with 5% non-fat milk (NFM) in TBST (Cell Signaling Technology 9997S), washed with TBST and incubation buffer (20 mM HEPES, 0.1 M KCl, 1 mM EDTA, 0.1 mM DTT, 10% glycerol,

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Tris Buffered Saline with Tween ® 20 (TBST-10X) from Cell Signaling Technology

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Tris Buffered Saline with Tween ® 20 (TBST-10X) from Cell Signaling Technology

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